Journal: Frontiers in Nutrition
Article Title: HMOS 2’FL and 3FL prevent house dust mite induced proinflammatory cytokine release in vitro and decrease specific IgE production in a murine allergic asthma model
doi: 10.3389/fnut.2025.1491430
Figure Lengend Snippet: T cell responses in lung tissue. Lung tissue of sacrificed mice was collected to determine the population of (A) activated T helper 2 cells based on the expression of T1ST2 and CD69 and the concentration of (B) IL13 in lung tissue as well as the population of (C) activated T helper 1 cells based on the expression of CXCR3 and CD69 and the concentration of (D) IFNγ in lung tissue. (E) Representative flow cytometry samples from Sham and HDM mice. When data fit a normal distribution and had an equal variability of differences, Sham and HDM groups are analyzed by unpaired t-test, the intervention groups were compared to the HDM group by One-Way ANOVA followed by a Dunnett’s multiple comparisons test. When these criteria were not met, data was transformed, a Mann-Withney test or Kruskal-Wallis test with Dunn’s multiple comparisons test was performed, n = 6–12, mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Article Snippet: Titrated amounts of the following antibodies were used to stain murine samples: CD4-BV510 (RM4-5, Biolegend), CD69-PE-Cy7 (H1.2F3, eBioscience), CXCR3-PE (CXCR3-173, eBioscience), T1ST2-FITC (DJ8, MD Bioproducts, USA), FoxP3-FITC (FJK-16 s, Invitrogen), CD127-PE/Vio770 (A7R 34, Miltenyi Biotech) and CD25-PerCP/Cy5.5 (PC61.5, Invitrogen).
Techniques: Expressing, Concentration Assay, Flow Cytometry, Transformation Assay